Plasmid_Backbone

Part:BBa_K1110000:Design

Designed by: Lydia Studdard   Group: iGEM13_Georgia_State   (2013-09-17)


pGAPzalphaA+RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3113
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3119
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3113
    Illegal BglII site found at 2354
    Illegal BamHI site found at 434
    Illegal XhoI site found at 3089
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3113
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3113
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3128
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1191
    Illegal NgoMIV site found at 1252
    Illegal AgeI site found at 153
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 417
    Illegal BsaI.rc site found at 616
    Illegal BsaI.rc site found at 1613
    Illegal SapI site found at 2597


Design Notes

Designed to conform to iGEM standards


Source

pGAPzalpha A, Saccharomyces cerevisiae

References