Plasmid_Backbone
Part:BBa_K1110000:Design
Designed by: Lydia Studdard Group: iGEM13_Georgia_State (2013-09-17)
pGAPzalphaA+RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3113
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3119 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3113
Illegal BglII site found at 2354
Illegal BamHI site found at 434
Illegal XhoI site found at 3089 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3113
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3113
Plasmid lacks a suffix.
Illegal XbaI site found at 3128
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1191
Illegal NgoMIV site found at 1252
Illegal AgeI site found at 153 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 417
Illegal BsaI.rc site found at 616
Illegal BsaI.rc site found at 1613
Illegal SapI site found at 2597
Design Notes
Designed to conform to iGEM standards
Source
pGAPzalpha A, Saccharomyces cerevisiae